产品名 | 脱氧核糖核酸酶Ⅰ(牛胰) |
别名 | 去氧核糖核酸酶;脱氧核糖核酸; 脱氧核糖核酸酶 ;胰道酶; 胰克氧核糖核酸酶; 除氧细胞核酸酶(牛胰); 去氧细胞核酸酶 |
英文名 | Deoxyribonuclease |
CAS号 | 9003-98-9 |
分子式 | C12H22N4O6 |
分子量 | 318.32628 |
EINECS编号 | 232-667-0 |
MDL号 |
https://onlinelibrary.wiley.com/doi/10.1002/adfm.202315275
【稳定性 Stability】
最佳的工作范围是 pH 7-8,DNase 的活性依赖于 Ca2+, 并可被二价金属离子如 Mn2+,Zn2+等激活。5mM Ca2+ 可保护酶使其不被水解,而 0.1mM Ca2+可使酶失活速 率降至原来的 1/2。
The optimal working range is pH 7-8, and the activity of DNase depends on Ca2+ and can be activated by divalent metal ions such as Mn2+, Zn2+, etc. 5mM Ca2+ can protect the enzyme from hydrolysis, while 0.1mM Ca2+ can reduce the enzyme inactivation rate to half.
【单位定义 Definition of unit】
以 1.5ml(0.15mg/ml 小牛胸腺 DNA)作底物,在 35℃且 pH5.0 的条件下,1 分钟内使反应液在波长 260nm 处的 吸光度增加 0.001 所需的酶量。(一个酶活力单位以 U 表示)
Using 1.5ml (0.15mg/ml calf thymus DNA) as substrate, the required enzyme amount is required to increase the absorbance of the reaction solution by 0.001 at a wavelength of 260nm within 1 minute under the condition of 35 ℃ and pH 5.0. (One enzyme activity unit is represented by U)
【产品特性 Properties】
1、 Mg2+存在条件下,DNase 可随机识别和切断 DNA 任一条链上的任意位点;而在 Mn2+存在条件下, DNase 识别 DNA 的两条链并在几乎相同的位点进 行切割;
Under the presence of Mg2+, DNase can randomly recognize and cleave any site on any strand of DNA; In the presence of Mn2+, DNase recognizes two strands of DNA and cleaves them at almost identical sites;
2、 DNase 可水解多种形式 DNA,如单链 DNA、双链 DNA,甚至染色质(其切割速率受组蛋白影响)。 DNase can hydrolyze various forms of DNA, such as single stranded DNA, double stranded DNA, and even chromatin (its cleavage rate is influenced by histones).
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